| Studies on biological characters and plasmid profiles of Escherichia coli isolated from pigs |
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Soo-kwan Jeong1, Suk-chan Jeong2, Won-pil Choi1 |
1College of Veterinary Medicine, Kyungpook National University
2Veterinary Research Institute, Rural Development Administration |
| 돼지 유래 대장균의 생물학적 특성과 plasmid profile에 대하여 |
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정수관1, 정석찬2, 최원필1 |
1경북대학교 수의과대학
2농촌진흥청 가축위생연구소 |
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| Abstract |
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The purpose of this study was the examination for presence of pilus antigen, O serogroups, colicin production, antibiotic susceptibility and plasmid profiles among E coli isolated from diarrheal piglets and fattening pigs in Taegu province. Of 145 E coli isolated, 98 strains (67.4%) possesed pilus antigens which belonged to either K88 (47.6%), K99 (11.7%) or 987P (8.3%) types. Fifty-nine strains (40.7%) were classified into tenO serogroups and their types were O8 (22.0%), O20(16.9%), O141(15.3%), O9(10.2%), O45(10.2%), O139(8.5%), O064(6.8%), O149(5.0%), O157(3.4%), and O115(1.7%). Thirty-three strains (22.8%) were colicinogenic and 6 strains (4.1%) were hemolytic. One hundred and thirty-nine strains (95.9%) of 145 E coli isolates were resistant to ampicillin, chloramphenicol, gentamicin, kanamycin, streptomycin, tetracycline, rifampicin and nalidixic acid, alone or in combination thereof. Ninety strains (64.7%) of 139 drug resistant strains carried R factor (R) which were transferable to the recipient by conjugation. In gel electrophoresis for the isolation of plasmid DNA, the number of plasmid DNA band varied from 2 to 11 in 16 E coli with pilus antigen. It's molecular weight ranged from 1.0 to 60.0 megadalton. |
| Key Words:
E coli, pilus, serotype, colicin, plasmid |
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