| Studies on nuclear transplantation in mouse embryos II. Developmental potential of nuclei from embryos of different developmental stages |
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Choong-saeng Park1, Sang-yong Choe2, Hyo-jong Lee2, Hee-sung Park1 |
1College of Agriculture, Gyeongsang National Uniνersity
2College of Veterinary Medicine, Gyeongsang National Uniνersity |
| 생쥐 수정란의 핵이식에 관한 연구 II. 발달단계별 수정란 핵의 이식후 생존성 |
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박충생1, 최상용2, 이효종2, 박희성1 |
1경상대학교 농과대학
2경상대학교 수의과대학 |
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| Abstract |
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Single nuclei from two-, four- and eight-cell mouse embryos were transplanted into enucleated two-cell embryos by micromanipulation and Sendai virus mediated fusion. The developmental potential of these reconstituted embryos in vitro and in vivo was examined. It was found that the single nuclei which were transplanted to enucleated two-cell embryos were not only able to develop to the blastocyst stage in vitro(two-cell nuclei, 76.5%; four-cell nuclei, 68.4%; eight-cell nuclei, 48.3%), but also able to develop to full term in vivo after transfer to recipient mice(two-cell nuclei, 37.1%; four-cell nuclei, 29.6%; eight-cell nuclei, 16.3%). Although the proportion of live young produced after transfer of nucler of nuclear transplant embryos which received eight-cell nuclei was significantly (p<0.05) reduced, it would be suggested that the overall efficiency in producing identical offspring is greater when eight-cell embryos were selected for nuclear donor than two- or four-cell embryos were selected. |
| Key Words:
nuclear transfer, in vitro deτelopment, embryo transfer, mouse embryo |
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