Studies on the electrophoretic analysis of serum proteins in dogs infected with Babesia gibsoni |
Dong-cheol Ihn, Joo-muk Lee, Joon-seok Chae, Hyeon-sung Cha |
College of Veterinary Medicine, Chonbuk National University |
Babesia gibsoni에 감염(感染)된 개의 혈청단백분획(血淸蛋白分劃)에 관한 연구 |
인동철, 이주묵, 채준석, 차현성 |
전북대학교 수의과대학 |
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Abstract |
To observe the changes of serum proteins according to the process of Babesia gibsoni(B gibsoni) infection, the babesia protozoa($10^8/kg$) were inoculated into the cephalic vein of healthy dogs. The serum proteins of experimentally infected dogs were separated by using cellulose acetate electrophoresis. The results obtained were as follows; 1. Cellulose acetate electrophoresis was fractionated to total 6 of bands such as, albumin, ${alpha}_1$, ${alpha}_2$, ${eta}_1$, ${eta}_2$ and $gamma$-globulin. 2. The concentration of total protein was shown a decreasing tendency after B gibsoni infection. Albumin and A/G ratio were lowered through all periods of the infection, but they were not significant changes. 3. The level of ${alpha}_1$-globulin was significantly(p<0.05) incresed in early stage of the infection. 4. The levels of ${alpha}_2$ and total $alpha$-globulin were shown highly significant decreases (p<0.01) through all periods of the infection. 5. The levels of ${eta}_1$ and total $eta$-globulin had highly significant changes (p<0.01) that was increased in early stage of infection and decreased later. 6. The level of $gamma$-globulin was seen to be constantly increased through all periods of infection. It was a highly significant change (p<0.01). 7. Plasma protein: fibrinogen (PP:F) ratio was shown a temporally significant increase (p<0.05) following the decrease in early infection. |
Key Words:
B gibsoni, cellulose acetate electrophoresis |
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