Effect of dietary vitamin E on the lipid peroxidation by dietary iron injected to male rats

Huh, Rhin-sou; Kim, Young-hong; Do, Jae-cheul; Choi, Yeon-shik; Cheong, Cheong-won

Korean Journal of Veterinary Research 1991;31(3):265-270.

Rhin-sou Huh, Young-hong Kim, Jae-cheul Do, Yeon-shik Choi, Cheong-won Cheong

College of Veterinary Medicine, Kyungpook National University

Vitamin E가 철분 투여로 인한 rat의 지질 과산화에 미치는 영향

허린수, 김영홍, 도재철, 최연식, 정정원

경북대학교 수의과대학

Abstract

This study was designed to investigate the effect of dietary vitamin E on the lipid peroxidation by dietary iron-injected to male rats. Sprague-Dawely strain male rats were divided into three experimental groups, namely control, iron injected and iron-vitamin E injected groups. The control group was fed with normal diet; the iron injected group was given normal diet and while injected intraperitoneally 30mg of ferric hydroxide/100g of body weight 20 times every 3 days. The iron-vitamin E injected group was intraperitoneally administered 30mg of ferric hydroxide/100g of body weight 20 times every 3 days and vitamin E every day with the dose of 5IU(5mg)/100g body weight. All experimental groups were maintained for 60 days with feeding on the respective ratio. The results obtained from this experiment were summarized as following: 1. The net weight gain was significantly decreased by the iron injection, but much increased by the vitamin E injection. 2. The contents of unsaturated fatty acid in phospholipid in liver, kidney, muscle and serum were decreased by the iron injection, but increased by the vitamin E injection. 3. The increment of malondialdehyde contents was induced by the iron overloading, but significantly decreased by the vitamin E injection. Therefore, it is suggested that dietary iron administration to male rats facilitates the lipid peroxidation in vivo and vitamin E has the inhibiting effect on lipip peroxidation process by iron.

Key Words: iron, vitamin E, unsaturated fatty acid, malondialdehyde, lipid peroxidation, antioxidative effect