Studies on the cloning gp50 and gp63 genes of Pseudorabies virus(Shope strain) |
Chang-hee Kweon1, Jae-young Song1, Byoung-han Kim1, Jung-bok Lee1, jae-chin Lee1, Soo-hwan An1, Yong-soon Lee2, Maeda Susumu3 |
1Veterinary Research Institute 2Seoul National University 3University of California, Davis |
Pseudorabies virus의 gp50과 gp63 유전자 클로닝에 관한 연구 |
권창희1, 송재영1, 김병한1, 이중복1, 이재진1, 안수환1, 이영순2, 3 |
1농촌진흥청 가축위생연구소 2서울대학교 수의과대학 3캘리포니아 대이비스 주립대학교 |
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Abstract |
The DNA fragment representing for Pseudorabies gp50 and gp60(Shope) was cloned by recombinant techniques. The viral DNA was extracted from the infected cells and digested with Bam HI. The 6.8 Kb of Bam HI fragment was isolated from agarose gel and further digested with Nde I followed by Klenow treatment. The blunt ended 4.9Kb fragment was cloned into pTZ18R plasmid vector. The upstream region of gp50 was further manipulated to remove its 5' promoter region and create EcoRl site for possible eukaryotic expression system. The result of partial sequencing of cloned DNA indicated that Shope strain showed 95% homology with gp50 of Rice strain. |
Key Words:
Pseudorabies virus, Shope strain, gp50 and gp63 |
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