Biophysical characteristics of a noncytopathic bovine viral diarrhea virus

Kweon, Chang-hee; Anthony, Castro E; Woo, Hee-jong

Korean Journal of Veterinary Research 1992;32(1):77-82.

Chang-hee Kweon¹, Castro E Anthony², Hee-jong Woo³

¹Veterinary Research Institute
²California Veterinary Diagnostic Laboratory(CVDLS) University of California Davis
³Laboratory of Cancer Biology, Harvard Medical School

세포 비병원성 소 설사병 바이러스의 이화학적 성상 조사

권창희¹, CastroEAnthony², 우희종³

¹가축위생연구소
²캘리포니아 수의진단 연구소
³하바드 의과대학

Abstract

A noncytopathic bovine viral diarrhea virus(NC BVDV) strain isolated and purified from persistently infected primary bovine fetal lung(BFL) cells was studied by biophysical methods. The buoyant density of particles of the NC BVDV strain was shown to be between 1,090 and $1,114g/cm^3$ and the maximum virus infectivity occured at $1,098g/cm^3$. Immunoelectron microscopic examination by using the partially purified virus revealed regular spherical particles 30~80nm in diameter. Differences in the genomic size of cytopathic and noncytopathic BVDV from infected cells were not found. A comparison of viral proteins of a noncytopathic and cytopathic strain(NADL) by immunoprecipitation using monoclonal antibody indicated that NC BVDV, compared to cytopathic NADL, was cell associated.

Key Words: Noncytopathic bovine viral diarrhea virus, fetal bovine lung cells, persistant infection buoyant density, Immunoelectron microscopy, cell associated characteristics