Comparison of soluble antigens from Leptospira interrogans serovars by SDS-PAGE, Crossed Immunoelectrophoresis and Immunoblotting

Baik, Yeong-ok; Mah, Jum-sool

Korean Journal of Veterinary Research 1992;32(2):195-205.

Yeong-ok Baik¹, Jum-sool Mah²

¹Cheil sugar & Co., LTD
²College of Veterinary Medicine Seoul National University

SDS-PAGE, Crossed Immunoelectrophoresis 및 Immunoblotting을 이용한 Leptospira interrogans 혈청형간 항원 비교

백영옥¹, 마점술²

¹제일제당주식회사
²서울대학교 수의과대학

Abstract

The soluble antigen profiles and antigenic specificities of Leptospira interrogans serovars icterhaemorrhagiae, canicola, pomona and hardjo were examined by SDS-polyacrylamide gel electrophoresis, crossed immunoeletrophoresis and immunoblotting. The profiles of protein, glycoprotein and fraction containing N-acetylglucosamine of 4 serovars were compared. The protein profiles of 4 serovars were very similar except the range of 14,400 to 30,000 daltons. Molecular weight of glycoprotein of L, pomona was lower than other serovars. L canicola showed extra N-acetylglucosamine bands having molecular weight of 82,000 and 90,000 daltons. In crossed immunoelectrophoresis, a close antigenic relationship was found between L icterohaemorrhagiae and L canicola. In immunoblottings conducted with soluble antigens and rabbit antisera of 4 serovars, Leptospira interrogans serovars possessed cross-reactive antigens and serovar-specific antigens. The molecular weights of serovar-specific antigens were 45,000, 82,000 and 90,000, 31,000 and 24,000 daltons in L icterohaemorrhagiae, L canicola, L pomona, and L hardjo, respectively.

Key Words: Leptospira interrogans, Soluble serovar-specific antigens, SDS-polyacrylamide gel electrophoresis, Immunoblotting, Crossed immunoelectrophoresis