Korean Journal of Veterinary Research 1992;32(3):377-380.
Evaluation of a cell enzyme-linked immunosorbent assay for the detection of Borna disease virus antibodies in experimentally infected animals
Du-sik Lee
Department of Veterinary Medicine, Cheju National University
보르나 바이러스를 실험감염시킨 동물에서 항체검출에 대한 세포효소면역반응법의 평가에 대한 연구
이두식
제주대학교 수의학과
Abstract
The value of the cell enzyme-linked immunosorbent assay as a possible replacement for the indirect immunofluorescence antibody test for the estimation of antibodies against BD virus was assessed in four animal models. The serum antibody response was measured by both assay systems;the variability of both tests was less than one diluent step, and correlation of the two tests was assessed using regression analysis. The study showed that the all four animal models gave satisfactory correlation of CELISA and IFA. There, CELISA is acceptable for use in mouse, rabbit, chicken and rat models.
Key Words: indirect immunofluorescense antibody test, cell enzyme-linked immunosorbent assay, Borna disease virus


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