Construction of recombinant DNA clone for bovine viral diarrhea virus

Yeo, Sang-geon; Cho, H.J.; Masri, S.A.

Korean Journal of Veterinary Research 1992;32(3):389-398.

Sang-geon Yeo¹, H.J. Cho², S.A. Masri²

¹College of Veterinary Medicine, Gyongsang National University
²Animal Diseases Research Institute

소 바이러스성 설사병 바이러스의 유전자 재조합 DNA clone의 작성에 관한 연구

여상건¹, ², ²

¹경상대학교 수의과대학
²

Abstract

Molecular cloning was carried out on the Danish strain of bovine viral diarrhea virus(BVDV) to construct strategy for the diagnostic tools and effective vaccine of BVD afterwards. A recombinant DNA clone(No. 29) was established successfully from cDNA for viral RNA tailed with adenine homopolymer at 3'-end. $^{32}P$-labeled DNA probes of 300~1,800bp fragments, originating from the clone 29, directed specific DNA-RNA hybridization results with BVDV RNA. Recombinant DNA of the clone 29 was about 5,200bp representing 41.6% of the full length of Danish strain's RNA, and restriction sites were recognized for EcoR I, Sst I, Hin d III and Pst I restriction enzymes in the DNA fragment.

Key Words: BVDV, gene cloning, DNA probe, DNA-RNA hybridization