Assessment of the fertilizing capacity of domestic animal spermatozoa by hamster test I. Comparison of storage temperatures for boar sperm and results of hamster test between boar and dog sperm

Kim, Yong-jun

Korean Journal of Veterinary Research 1992;32(3):435-450.

Yong-jun Kim

College of Veterinary Medicine Chonbuk National University

Hamster test를 이용한 가축정자(家畜精子)의 수정능력(受精能力) 검정(檢定) 1. 돼지정자의 보존온도(保存溫度) 비교 및 돼지와 개정자의 hamster test결과

김용준

전북대학교 수의과대학

Abstract

To evaluate the fertilizing capacity of domestic animal spermatozoa by hamster test, semen were collected from 15 boars(Duroc, Landrace, and Yorkshire) and 2 mixed dogs which had been proved to be fertile in the past then, the semen were preserved in BWW medium at $4^{circ}C$ or $18^{circ}C$ for about 20 hours and coincubated with zona-free hamster ova for 5 hours. The ova were stained by lacmoid and examined under phase contrast microscope to investigate the rates of sperm binding to the ova, penetration and formation of a male pronucleus, and the numbers of both bound and penetrated sperm per ovum. Both the semen preserved at $18^{circ}C$ for about 20 hours and that treated by swim up procedure showed considerably higher rates of sperm binding and penetration as well as higher number of penetrated sperm than that preserved at $4^{circ}C$ for about 20 hours, respectively(p<0.01). Motility of boar sperm at insemination was from 40 to 90% and no difference in hamster test was obtained according to different degree of sperm motility. Abnormality in morphology of boar sperm at insemination was from 6 to 45% and no difference in hamster test was obtained according to different degree of sperm abnormality. The sperm concentrations of $7{ imes}10^7$ and $7{ imes}10^6$ showed considerably higher rates of sperm binding and penetration as well as higher number of bound sperm than that of $7{ imes}10^4$ (p<0.01) along with the same higher results than that of $7{ imes}10^5$(0<0.05), respectively. Boar sperm showed considerably higher rates of sperm binding and penetration as well as higher numbers of both bound and penetrated sperm than dog sperm, when both semen were treated by BWW＋heparin medium and swim up procedure, respectively. These results indicated that fertile boar sperm showed considerably lower rates in the results of hamster test, when preserved at $4^{circ}C$ for about 20 hours and in lower concentration of sperm than when preserved at $18^{circ}C$ for about 20 hours and in higher concentration of sperm, respectively, and at the same time considerably higher results than fertile dog sperm, consequently to prove that hamster test would be of great value in assaying the fertilizing capacity of boar sperm.