| Parthenogenetic development of mouse eggs I. Parthenogenetic activation by ethanol and hyaluronidase treatments |
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Hyo-jong Lee1, Dae-sik Ha2, Tae-young Kang1, Min-cheol Choi1 |
1College of Veterinary Medicine, Gyeongsang National University
2Kyongsangnam-Do Provincial Government Institute of Health and Environment |
| 생쥐 난자의 단위발생에 관한 연구 I. Ethanol 및 hyaluronidase처리에 의한 단위발생유기 |
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이효종1, 하대식2, 강태영1, 최민철1 |
1경상대학교 수의과대학
2경상남도 보건환경연구원 |
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| Abstract |
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This experiment was carried out to find out the best condition for the parthenogenetic activation of mouse eggs by treating ethanol and hyaluronidase. For the parthenogenetic activation of eggs with ethanol, cumulus cell enclosed or denuded eggs were treated with 7% ethanol in D-PBS for 5, 7 or 9 minutes. For the activation of eggs with hyaluronidase, the eggs with cumulus masses were released into D-PBS with 100 unit hyaluronidase and treated for 10, 12 or 13 minutes. All of the treated eggs were incubated in BMOC-3 solution for 5 hours at $37^{circ}C$ at an atmosphere of 5% $CO_2$ in air. The types of parthenogenetic eggs were morphologically classified into haploid, diploid, immediate cleavage eggs under an inverted microscope. The results obtained in this experiment were summarized as follows ; 1. High activation rate(99%) had been achieved by treating the eggs with 7% ethanol for 7 minutes. 2. With 100 IU hyaluronidase, high activation rate (94%) had been achieved by treating for 12 minutes. 3. The most frequent type of parthenogenetic eggs activated with ethanol or hyaluronidase was haploid (p<0.05). 4. The eggs collected from 18 to 22 hours post HCG injection showed higher activation rate than the eggs collected at 16 hours post HCG injection. 5. No significant difference (p>0.05) in activation rate was shown in strain of mouse and in presence of cumulus cells. |
| Key Words:
parthenogenesis, ethanol, hyaluronidase, mouse egg |
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