Antibiotics produced by anaerobic fermentation of Streptococcus sp. An-21-1 isolated from domestic soil I. Screening and identification of anaerobic bacteria

Park, Seung-chun; Yun, Hyo-in; Oh, Tae-kwang

Korean Journal of Veterinary Research 1993;33(1):53-60.

Seung-chun Park¹, Hyo-in Yun¹, Tae-kwang Oh²

¹Institute of Vet. Sci., College of Vet. Med., Chungnam National University
²Genetic Engineering Research Institute, KAIST

국내토양에서 분리한 혐기성 세균 Streptococcus sp. An-21-1 이 생성하는 항생물질 I. 혐기성 세균의 선별과 동정

박승춘¹, 윤효인¹, 오태광²

¹충남대학교 수의과대학 동물의과학연구소
²한국과학기술원 유전공학연구소

Abstract

Anaerobic bacteria are suggested to be potential source for new antibiotics. In order to search for antibiotics from domestic origin, we collected 800 soil samples across Korean locations and could isolate as many as 989 anaerobic strains. Among them 10, strains were found to have good producing capacity of antibiotics. An anaerobe was finally selected due to secreting antibiotics having high antimicrobial activity towards multiple resistant microorganism(E coli JM 83) transformed by genetic engineering technique. Its morphological, physiological and biochemical charateristics were investigated, together with antimicrobial spectrum therefrom. On antimicrobial spectrum study, substance secreted from this strain, had no activities to fungus and yeast. The selected strain showed G(+) and coccal shape, on Gram, staining and electron scanning microscopy, respectively. Biochemically this strain utilized glucose, fructose lactose, sucrose, but did not arabinose, cellulose, rhamnose, sorbitol, trehalose, mannitol. Catalase test showed negative property. Optimal growth temperature was $37^{circ}C$. The results obtained above suggest this strain Streptococcus faecium subspp. and we named it Streptococcus sp. An-21-1.

Key Words: anaerobes, antibiotics, E coli JM 83, domestic soil, Streptococcus sp. An-21-1