| Acridine orange-induced changes in the O-side chain lengths of lipopolysaccharides of Salmonella pullorum |
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Jong-bae Kim, Tae-ue Kim, Won-yong Lee, Se-whan Yang |
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Department of Medical Technology, College of Health Science, Yonsei University |
| Salmonella pullorum lipopolysaccharide의 acridine organe에 의한 O-side chain 길이의 변화 |
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김종배, 김태우, 이원용, 양세환 |
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연세대학교 보건과학대학 임상병리학과 |
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| Abstract |
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The morphological hetergeneity of lipopolysaccharides(LPSs) and variation in the O-side chain lengths of LPSs of Salmonella pullorum, which was serially subcultured on the brain heart infusion agar containing $100{mu}g/m{ell}$ of acridine orange, was analyzed in Sephadex G-50 column chromatography and silver-stained polyacrylamide gels. The biochemical differences in LPS W and LPS A0150 were identified. Increases in the contents of O-polysaccharides of LPS A0150 than those of LPS W were reflected in the profiles of chromatography and silver-stained polyacrylamide gels. In summary, LPS molecules of S pullorum A0150 appeared to be enriched in the molecules with long O-polysaccharide chains than those of LPS W. |
| Key Words:
S pullorum, lipopolysaccharide, O-side chain, acridine orange |
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