| Enzyme-linked immunosorbent assay for detection of bovine antibody to Brucella abortus |
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Yoon-kyu Lim1, Doo-sick Lee1, Jun-hong Park1, Ki-chun Yang1, Seung-ho Kim1, Kong-sick Kim2, Kwan-jong Hyun2, Woo-tack Kim2, Yong-soon Lee3 |
1Department of Veterinary Medicine, Cheju National University
2Cheju Veterinary Service Laboratory
3CoIlege of Veterinary Medicine, Seoul National University |
| 축우 부루셀라병의 ELISA 진단법에 관한 연구 |
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임윤규1, 이두식1, 박전홍1, 양기천1, 김승호1, 김공식2, 현관종2, 김우택2, 이영순3 |
1제주대학교 수의학과
2제주도가축위생시험소
3서울대학교 수의과대학 |
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| Abstract |
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Enzyme-linked Immuno sorbent Assay (ELISA) for the serological diagnosis of Brucella abortus was developed and compared with plate agglutination test. Cell wall antigen was extracted from Brucella abortus 1119-3 by sonication and with a sodium deoxychlate solution. Optimum protein concentration of coating antigen were $0.4{mu}g/100{mu}{ell}$ protein on each microtiter plate well. Horse radish peroxidase (HRP) labeled protein-G was used as a tracer of reacted antibodies. ELISA confirmed the agreeable results of 40 cases out of 43 cases by plate aggulutination test. ELISA diagnosed positive cases(10 out of 12) and negative cases (1 out of 12) with dubious sera by plate agglutination test. From this results ELISA could be used for the early diagnostic tools of Brucellosis in cattle. |
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