Isolation of porcine epidemic diarrhea virus (PEDV) in Korea

Kweon, Chang-hee; Kwon, Byung-joon; Jung, Tae-sung; Kee, Young-jin; Hur, Dong-ho; Hwang, Eui-kyung; Rhee, Jae-chin; An, Soo-hwan

Korean Journal of Veterinary Research 1993;33(2):249-254.

Chang-hee Kweon, Byung-joon Kwon, Tae-sung Jung, Young-jin Kee, Dong-ho Hur, Eui-kyung Hwang, Jae-chin Rhee, Soo-hwan An

Veterinary Research Institute

돼지 유행성 설사 바이러스 (porcine epidemic diarrhea virus)의 국내 분리주 작성에 관한 연구

권창희, 권병준, 정태성, 기영진, 허동호, 황의경, 이재진, 안수환

가축위생연구소

Abstract

The etiological survey on porcine epidemic diarrhea virus(PEDV) by immunofluorescence antibody test(IFA) showed the positive rusult from the intestines of piglet died from acute diarrhea. The viral agent of PED was also isolated from intestine, which showed positive reaction by immunofluorescence test. After passage in Vero cell, the viral agent was further cloned by plaque purification and designated as KPEDV-9. The immunoblotting analysis using hyperimmune sera and porcine sera revealed the presence of several polypetide bands with molecular weight(M.W.) of 88K, 74K, 70K, 58~54, 54~46K, 44~40K and 33~32K, respectively.

Key Words: porcine epidemic diarrhea virus, derivation of KPED-9 strain, immunoblotting analysis