Preservation of Toxoplasma gondii (RH strain) in liquid nitrogen for 5 years

Jeon, Yeong; Jang, Hwan

Korean Journal of Veterinary Research 1993;33(2):285-287.

Yeong Jeon, Hwan Jang

Veterinary Research Institute

톡소플라즈마원충(原蟲)(RH주(株))의 5년간 동결보존시험

전영, 장환

가축위생연구소

Abstract

Toxoplasma gondii frozen by the method of very slow cooling ($-30^{circ}C/3hrs.{ ightarrow};-60^{circ}C/lhr.{ ightarrow};-196^{circ}C$ of liquid nitrogen)were inoculated mice after storage in the liquid nitrogen for 5 years. The results obtained were as follows; 1. Toxoplasma gondii was detected in mice 4~5th day after intraperitoneal inoculation of organisms stored in the liquid nitrogen once a year for 5 years. 2. Toxoplasma gondii could be preserved longer than 5 years in liquid nitorgen being evidenced by the in vivo test.

Key Words: mouse, Taxoplasma gondii(RH strain), liquid nitrogen