Korean Journal of Veterinary Research 1993;33(2):345-350.
Effects of glycerol and seminal plasma in characteristics of preserved canine spermatozoa
Kye-seong Kim
College of Veterinary Medicine, Seoul National Universtiy
개 정액의 보존시 glycerol 첨가 및 정장제거가 정자의 성상에 미치는 영향
김계성
서울대학교 수의과대학
Abstract
Multiple ejaculates were collected from four male mongrel dogs. The second fraction and the small volume of third fraction from the ejaculates were divided and treated as follows : control; addition of the egg-yolk Tris extender to the semen at $37^{circ}C$. group I; Removal of seminal plasma, group II; addition of the glycerolated extender at $4^{circ}C$, group III Removal of seminal plasma and addition of glycerolated extender at $4^{circ}C$. The semen cooled to $4^{circ}C$ was equlibrated for 2hrs and preserved in refrigerator at $4^{circ}C$. The preserved semen was evaluated for kinetics, morphology, motility and thermoresistance daily for 3 days. 1. The kinectics after preserved days 2 and 3 of group I was significantly higher than that of control(p<0.05). 2. There were no significant difference in abnormal morphology of each group between the periods of storage. 3. The motility after preserved day 1 and days 3 of group I was significantly higher than that of others(p<0.05), and the molity after preserved days 2 of group I and III was signficantly higher than that of others(p<0.05). 4. When the molity of preserved semen was measured during incubation at $37^{circ}C$, the motility of four groups was declined at similar rates. There was no effect of removal of seminal plasma and glycerol addition on thermoresistance.
Key Words: canine spermatozoa, glycerol, seminal plasma, preservation


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