Effect of filtrates of Serpulina(Treponema) hyodysenteriae on the epithelium in colonic loops in swine |
Boo-hyon Kang1, Harvey J Olander2 |
1Toxicology Research Center, Korea Research Institute of Chemical Technology 2Department of Pathology, School of Veterinary Medicine, University of California |
Serpulina(Treponema)hyodysenteriae 배양여과액의 돼지 공장 계제 점막에 미치는 영향 |
강부현1, 2 |
1한국화학연구소 안전성연구센터 2California, Davis 수의과대학 |
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Abstract |
The cytotoxic effects of S hyodysenteriae strain B 204 on the mucosal surface were studied in surgically prepared ligated colonic loops in two male convenitonal mixed-breed pigs. In each one of four loops was inoculated with either sterile trypticase soy broth(TSB) of Serpulina, filtrate of Serpulina TSB culture. washed Serpulina cells or whole culture of Serpulina. Mucosal tissues were examined by transmission and scanning electron microscopy 24 and 48 hours after inoculation(p.i.). The filtrate did not induce any significant effect on the mucosal surface. The washed cells produced early lesions similar to those caused by the whole culture. These observations suggest that cytotoxins of the culture do not play a significant role in invasion of the epithelium in this experimental infection. The possible role of toxins associated with the organism at the site of interaction with the epithelial cells was not elucidated. |
Key Words:
swine, Serpulina hyodysenteriae, TEM, SEM, intestine, cytotoxin |
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