| The effect of captafol on the hematological value, erythrocyte membrane and plasma biochemical value in vitro |
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Sung-hoon Kim, Hwa-seon Hwang, Shin-woo Cha, Sang-seop Han, Jung-koo Roh |
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Toxicology Research Center Korea Research Institute of Chemical Technology |
| 시험관내에서 captafol이 혈액학, 적혈구막 및 혈장생화학치에 미치는 영향 |
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김성훈, 황화선, 차신우, 한상섭, 노정구 |
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한국화학연구소 안전성연구센터 |
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| Abstract |
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The effect of captafol on the hematological value, erythrocyte membrane and plasma biochemical value was investigated using blood of SPF Sprague-Dawley male rats in vitro. For the anticoagulations, we used 0.5mg of heparin per $10m{ell}$ of blood from vena cava. Three con-centrations($0.1{ imes}10^{-4}M$, $1{ imes}10^{-3}M$ and $1{ imes}10^{-2}M$) captafol in ethanol were added to the each $2.5m{ell}$ blood so that the linal concentration of ethanol was 1%. The blood contained with each concentration of captafol was incubated at $37^{circ}C$ C for 2 hours under 5% $CO_2$ gas The whole blood and plasma were examined for hematological vaJues, erythrocyte membrane damage and biochemical values, respectively. The results obtained were summarized as follows ; 1. The number of RBC in $1{ imes}10^{-2}M$ group and the concentration of MCHC in $1{ imes}10^{-3}M$ group were significantly (p<0.05) decreased and increased from that of control values, respectively. The percentage of Hct was significantly (p<0.05) decreased with dose-response. 2. Erythrocyte fragility rate of $1{ imes}10^{-3}M$ and $1{ imes}10^{-2}M$ group were significantly (p<0.01) increased from that control with dose response. 3. Potassium ion level of $1{ imes}10^{-2}M$ was significantly (p<0.05) increased from that of control. 4. The concentration of total bilirubin in the $1{ imes}10^{-3}M$ and $1{ imes}10^{-2}M$ groups were significantly increased from that of control. The enzyme level of creatine kinase in $1{ imes}10^{-2}M$ group was significanlty (p<0.05) decreased from control value. |
| Key Words:
captafol, hematological value, erythrocyte membrane, plasma biochemical value, in virto |
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