Korean Journal of Veterinary Research 1993;33(4):605-609.
Active metabolites in rat bile after intravenous injection of [3H] pteroylglutamic acid
Ho-chul Shin, Minoru Shimoda, Eiichi Kokue
Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology
랫드에 있어서 [3H] pteroylglutamic acid 의 정맥주사후(靜脈注射後) 담즙중(膽汁中) 활성대사물(活性代謝物)에 관한 연구(硏究)
신호철, 시모다미노루, 코쿠에에이치
동경농공대학 수의학과
Abstract
Active metabolites in rat bile after an intravenous injection of $[^3H]$ pteroylglutamic acid(PteGlu)were studies using high-performance liquid chromatography(HPLC). Predominant four radioactive metabolites and parent compound PteGlu were detected on the chromatogram of HPLC with liquid scintillation counting system. These metabolites were identified as tetrahydrofolate, 10-formyltetrahydrofolate, 5-methyltetrahydrofolate and para-aminobenzoyl glutamate. The identification of active folate metabolites was based on the consistency of retention time profiles and hydrodynamic voltammograms which were obtained by HPLC with the electrochemical detection system, and characteristics of UV absorbance spectra obtained by HPLC with photodiode array detection system.
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