Korean Journal of Veterinary Research 1993;33(4):611-615.
Development of an enzyme immunoassay for determination of steroid hormones to improve the reproductive efficiency of domestic animals
Han-sun Choi, Byong-kyu Kang
College of Veterinary Medicine, Chonnam National University
가축(家畜)의 번식효율증진(繁殖效率增進)을 위한 steroid hormones 의 효소면역분석법(酵素免疫分析法) 개발(開發)
최한선, 강병규
전남대학교 수의과대학
Abstract
A rapid, solid-phase microtitre plate enzyme immunoassay(EIA) to determine the concentration of progesterone and testosterone in dairy cow is described. Both steroid hormones were analysed employing antibodies against $11{alpha}$-hemisuccinate-progesterone bovine serum albumin and 4-androsten-$17{eta}$-ol-3-one-carboxymethyloxime bovine serum albumin, respectively. as primary antibodies and sheep Ig G as secondary antibody. The conjugated used as labels for progesterone and testosterone was progesterone-$11{alpha}$-hydroxy-hemisuccinate- horseradish peroxidase and 4 ${alpha}$-androsten-$17{eta}$-ol-3-hemisuccinate- horseradish peroxidase, respectively. Detection limit of microtitre plate EIA was 6.7 pg/well for progesteone and 1.0 pg/well for testosterone.
Key Words: enzyme immunoassay, progesterone, testosterone


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