Serum cortisol concentrations on the normal condition in Korean native goats by ELISA

Cho, Kyu-woan; Hur, Ju-hyeong; Lee, Eun-sug; Kang, Chung-boo

Korean Journal of Veterinary Research 1993;33(4):711-717.

Kyu-woan Cho, Ju-hyeong Hur, Eun-sug Lee, Chung-boo Kang

College of Veterinary Medicine, Gyeongsang National University

ELISA에 의한 국내 재래종 산양의 정상상태에서의 혈중 cortisol 농도

조규완, 허주형, 이은석, 강정부

경상대학교 수의과대학

Abstract

This study was performed to examine the charges of serum cortisol concentrations on the normal conditions in Korean native goats by enzyme-linked immunosorbent assay(ELISA). The Korean native goats were 4 to 30 months old and weighted 6.0 to 28kg. The goats were allowed ad libitum access to water and food. To minimize the stress with handing and blood sampling, animals were adapted for 2 weeks before the experiment. After adaptation, intravenouse catheter was inserted left intact a jugular vein of 6 goats and blood samples were done 2~3 days later. Experimental animals were divided into 2 groups which was non-catheterized and catheterized group in order to examine the effect for changes of serum concentration and circadian rhythm of cortisol. The results were obtained as follows ; The sensitivity of serum cortisol concentration was $20pg/m{ell}$. The intra-assay and inter-assay coefficient of variation were below 2.5% and 5%, respectively. Serum concentration of cortisol was more higher in noo-catheterized group$(32.84{pm}16.78ng/m{ell})$ than catheterized group$(23.20{pm}10.29ng/m{ell})$. The difference according to months old in serum concentration of cortisol was the more higber on 4~6 than 7~12 and lowest over 12 months old. A circadian rhythm in the serum concentration of cortisol in Korean native goats was not found significantly with 2 hours sampling intervals.

Key Words: cortisol, goat, ELISA, circadian rhythm