Isolation of porcine reproductive and respiratory syndrome virus(PRRSV) in Korea

Kweon, Chang-hae; Kwon, Byung-joon; Lee, Han-jung; Cho, Jae-jin; Hwang, Eui-kyung; Shin, Jin-ho; Yoon, Yong-dhuk; Kang, Yung-bai; An, Soo-hwan; Kim, Yong-hee; Huh, Won; Jun, Moo-hyung; Wensvoort, G.

Korean Journal of Veterinary Research 1994;34(1):77-83.

Chang-hae Kweon¹, Byung-joon Kwon¹, Han-jung Lee¹, Jae-jin Cho¹, Eui-kyung Hwang¹, Jin-ho Shin¹, Yong-dhuk Yoon¹, Yung-bai Kang¹, Soo-hwan An¹, Yong-hee Kim², Won Huh³, Moo-hyung Jun⁴, G. Wensvoort⁵

¹Veterinary Research Institute
²Bayer Veterinary Medical Research Institute
³Daesung Laboratory
⁴College of Veterinary Medicine, Chungnam National University
⁵Central Veterinary Institute

돼지생식기 및 호흡기증후군(Porcine Reproductive and Respiratory Syndrome ; PRRSV) 바이러스의 국내분리주 작성에 관한 연구

권창희¹, 권병준¹, 이한정¹, 조재진¹, 황의경¹, 신진호¹, 윤용덕¹, 강영배¹, 안수환¹, 김용희², 허원³, 전무형⁴, ⁵

¹가축위생연구소
²한국 바이엘 동물의약연구소
³대성 미생물연구소
⁴충남대학교 수의과대학
⁵네덜란드 중앙수의연구소

Abstract

Three viral strains, causing CPE in porcine alveolar macrophage cell, were isolated from aborted fetus, serum from young pig showing blue-ear sign and lung of suspected pig, respectively. The differential diagnostic results showed no characteristics of Aujeszky's disease virus(ADV), hog cholera virus (HCV), Japanese encephalitis virus(JEV), porcine parvovirus(PPV) and encephalomyocarditis virus (EMCV). However, positive reactions were demonstrated by IFA using monospecific porcine antibodies against Lelystad virus. When the paired sera of experimentally inoculated swine with one of isolate, KPRRSV-l were tested by IPMA, the result indicated that the isolate was related to United States isolate than European LV.

Key Words: Porcine reproductive and respiratory syndrome, isolation of KPRRSV strains, antigenic relationship, Lelystad virus, United States isolate