Identification of Staphylococcus hyicus subsp hyicus of swine and poultry origin by API STAPH-IDENT system

Park, Cheong-kyu

Korean Journal of Veterinary Research 1994;34(2):315-320.

Cheong-kyu Park

College of Veterinary Medicine, Kyungpook National University

API STAPH-IDENT system에 의한 돼지 및 닭유래(由來) staphylococcus hyicus subsp hyicus의 동정(同定)

박청규

경북대학교 수의과대학

Abstract

The API STAPH-IDENT system was evaluated as a means for identifying Staphylococcus hyicus subsp hyicus straints isolated from swine and poultry. Of 80 strains from swine, 68 (85%) were correctly identified by the API STAPH-IDENT system alone after 5 h of incubation. When results were determined after 24 h of incubation, the accuracy of this system alone was 93.8%. By additional tests in conjunction with the API STAPH-IDENT system, however, all 80 strains could be correctly identified. Of 120 strains from poultry, 87 (72.5%) required additional testing to achieve a correct identification, and 33 (27.5%) were incorrectly identified by this system after 5 h of incubation. After 24 h of incubation, 99 of 120 (82.5%) avian strains were incorrectly identified as Staph epidermidis owing to false-negative mannose and trehalose utilizations. Seventy-seven (96.3%) of swine strains were positive for ${eta}-glucuronidase$, whereas all 120 strains recovered tram poultry were negative for it.

Key Words: Staphylococcus hyicus, swine, poultry, API STAPH-IDENT system, identification, ${eta}-glucuronidase$