| The effects of exogenous insulin-like growth factor-I on broiler chicken growth |
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Ho-il Lee1, Moon-joon Lee2, Dae-yeoul Lee3, Young-ann Kim4, Chang-won Kang1, Seung-ki Chon1 |
1College of Veterinary Medicine, Chonbuk National University
2Iri Agricultural and Technical College
3Medical school, Chonbuk National University
4Faculty of pharmacy, Woosuk University |
| 외인성 insulin-like growth factor-I이 육계의 성장에 미치는 영향 |
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이호일1, 이문준2, 이대열3, 김영안4, 강창원1, 전승기1 |
1전북대학교 수의과대학 수의 생리학교실
2이리 농공 전문 대학
3전북대학교 의과대학 소아과학 교실
4우석대학교 약학부 |
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| Abstract |
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Insulin-like growth factor-I(IGF-I) plays an important role in the regulation of mammalian and poultry growth. IGF-I has many actions in different tissues, which include metabolic, mitogenic, and differentiative actions. IGF-I induces insulin-like effects - such as increased cell glucose uptake and glycogen sysnthesis, however several physiological actions of IGF-I may not have been identified yet. In order to investigate the effect on growth in broiler chicken treated with exogenous insulin-like growth factor-I, 30 chickens were injected $50{mu}g$ reconbinant human IGF- I (rhIGF- I ) per kg body weight as experimental group and 30 ckickens saline subcutanously as control, 3 times according to ages from 2 to 35 days. We established radioimmunoassay method by which we can measure chicken IGF- I (cIGF- I ) as in rhIGF- I assay. The results obtained were as follows; 1) The dilution curve showed in parallelism between rhIGF- I and cIGF- I in the Sep-pak $C_{18}$ cartridge plasma extracts. 2) The body weight of broiler chicken were significantly increased at 31 days($1,176.50{pm}99.79g$) and 35 days($1,252.84{pm}125.21g$) of age in treatment groups, compared with control group($1,011.88{pm}40.22g,;1,111.32{pm}153.67g$). The liver and kidney weights on 35 days$(35.24{pm}5.18g,;11.05{pm}1.47g)$ were significantly higher in rhIGF- I treated group than control group($30.95{pm}4.04g,;10.01{pm}1.60g$) 3) The plasma concentration of IGF- l and total protein in rhIGF- I treated group were $58.17{pm}1.69ng/ml$, $3.75{pm}0.62g/dl$ respectively compared with control group $45.70{pm}1.64ng/ml$, $2.32{pm}0.53g/dl$. The results suggest that exogenous rhIGF- I increased total body weight, liver and kidney weights in broiler chicken, and it may increase IGF- I and total protein concentration in serum. |
| Key Words:
IGF- I, broiler chicken growth, liver, RIA, kidney, protein |
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