Morphological studies on kidney in cyclophosphamide-treated NOD mice

Lee, Joon-sup; Seong, Je-kyung

Korean Journal of Veterinary Research 1994;34(4):653-664.

Joon-sup Lee, Je-kyung Seong

College of Veterinary Medicine, Seoul National University

Cyclophosphamide를 투영한 NOD 마우스의 신장에 대한 형태학적 연구

이준섭, 성제경

서울대학교 수의과대학

Abstract

This study was carried out to investigate diabetic renal changes in cyclophosphamide(CY) treated nonobese diabetic(NOD) mice and to develop animal model of diabetic human nephropathy. The 8-week-old NOD and ICR mice were injected with cyclophosphamide intraperitoneally at 200mg/kg body weight and compared the chemical effects on these mice with the non-treated NOD and ICR mice respectively. The renal glomeruli in ICR, cyclophosphamide-treated ICR(ICR+CY), NOD and cyclophosphamide-treated NOD(NOD+CY) mice were observed by the light and electron microscopes. The results obtained were summarized as follows ; 1. Spontaneous incidences of diabetes mellitus in NOD mice were significantly promoted by dosing with cyclophosphamide. 2. Glomerulohypertrophy, proliferation of mesangium, partial thickening of glomerular basement membrane, and partial fusion of pedicels of podocyte were observed in NOD mice and NOD+CY mice. These changes were not observed in both ICR mice and ICR+CY mice. 3. The diabetic nephropathy observed in NOD+CY mice was more severe than that of non-treated NOD mice.

Key Words: NOD mouse, diabetes mellitus, renal glomerulus, cyclophosphamide