| Role of obioactin on toxoplasmacidal activity within mouse peritoneal macrophages |
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Mhan-pyo Yang |
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College of Veterinary Medicine, Chungbuk National University |
| 마우스 복강 macrophages내(內)의 살(殺)톡소플라즈마 활성에 있어서 obioactin의 역할 |
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양만표 |
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충북대학교 수의과대학 |
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| Abstract |
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The present study was undertaken to examine the effects of obioactin, lonomycin A, and MDP on toxoplasmacidal activities in glycogen-induced mouse peritoneal macrophages. The killing effect of obioactin on Toxoplasma multiplication was increased significantly in proportion to its concentrations. $O_2{^-}$ generation in obioactin-treated macrophages was also increased from twofold to threefold when compared with that of untreated control. Similarly, $H_2O_2$ continued to rise in parallel with increase of the concentration of obioactin. Lonomycin A-treated macrophages also exhibited a good effect of dose-response on toxoplasmacidal activities. However, $O_2{^-}$ and $H_2O_2$ were not generated significantly in lonomycin A-treated macrophages. Macrophages treated with muramyl dipeptide (MDP) were not found to inhibit the prolifi:ration of Toxoplasma but showed the enhancement of $O_2{^-}$ and $H_2O_2$, generation. The released lysozyme levels from macrophages into cultured media were decreased tn dose-dependent fashion by in vitro treatment of obioactin, lonomycin A, and MDP. The intracellular lysozyme levels appeared to be a constant value regardless of increasing the concentrations of obioactin, lonomycin A, and MDP. Therefore, these results suggest that Toxoplasma multiplication within macrophages treated with obioactin was inhibited by the generation of $O_2{^-}$ and $H_2O_2$ and that lysozyme per se within or released from macrophages had no effect on toxoplasmacidal activity. |
| Key Words:
hydrogen peroxide, macrophage, obioactin, superoxide anion, Toxoplasma |
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