| Analysis of human HoxA gene control region and its effects on anterior-posterior axial pattern formation using transgenic mouse embryo |
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Seung-ik Jang1, Won-gi Min2, Jong-hoon Park2, Chul-sang Lee2, Kyung-kwang Lee2, Young-won Lee2, Moo-hyung Jun1, Myoung-hee Kim2 |
1College of Veterinary Medicine, Chungnam National University
2Genetic Engineering Research Institute, KIST |
| Transgenic mouse embryo를 이용한 human HoxA 유전자의 조절부위 분석과 전후축 형태형성(anterior-posterior axial pattern formation)에 미치는 영향 |
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장승익1, 민원기2, 박종훈2, 이철상2, 이경광2, 이영원2, 전무형1, 김명희2 |
1충남대학교 수의과대학
2한국과학기술연구원 유전공학연구소 |
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| Abstract |
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The human homolog of position specific element of mouse Hoxa-7 was studied using transgene. It contains a 1.1 kb human DNA (HCR)- a homolog to the intergenic region between Hoxa-7 and -9, which directs the position specific expression of Hoxa-7-, tk promoter, LacZ (${eta}$-galactosidase) gene as a reporter, and polyadenylation signal of SV40 large T antigen. It was injected into the mice embryos, and the resulting transgenic embryos were analysed through PCR as well as genomic Southern blotting with placenta DNA. Out of 20 embryos analysed, two were transgenic. Among them, one transgenic embryo expressed transgene when stained with X-gal. The expression pattern was in analogy to that of the mouse Hoxa-7, showing spatially restricted expression pattern, Since the expression of ${eta}$-galactosidase is regulated by the upstream human HCR sequence, it implies that the HCR is the plausible position specific regulatory element of human. |
| Key Words:
human, HOXA, control region, X-gal staining, transgenic mouse embryo |
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