Studies on the culture of bovine nuclear transplant embryos derived in vitro fertilization

Hwang, Woo-suk; Jo, Choong-ho

Korean Journal of Veterinary Research 1995;35(1):179-185.

Woo-suk Hwang, Choong-ho Jo

College of Veterinary Medicine, Seoul National University

체외수정 유래 소 핵이식란의 배양에 관한 연구

황우석, 조충호

서울대학교 수의과대학

Abstract

The fusion rates of nuclear transplant embryos with various DC voltage were 55.6-79.2%. The significantly higher fusion rates of nuclear transplant embryos were achieved at the electric field strenght of DC 1.0-2.0kV/cm(72.0-79.2%) than DC 0.75kV/cm(55.6%, P<0.05). No significant differences in the percentage of embryos that cleaved(48.1, 55.4 and 42.6% respectively) and the percentage of cleaved embryos that developed to morulae/blastocyst(1.9, 5.3 and 1.9% respectively) could be found among the three types of in vitro culture system (Granulosa cell, BOEC co-culture and SOF, P>0.01). The age of the recipient cytoplast(30 vs 40hr) had no effect on the fusion rates and the rates of cleavage development(36.9 vs 44.1%, P>0.01).

Key Words: bovine, nuclear transfer, electrofusion, in vitro development