Studies of the chromosome structure of tissue cultured different organisms from the rainbow trout |
Yung-rae Jung1, Young-gil Jeong1, Jin-ki Son2, Moo-kang Kim1 |
1College of Veterinary Medicine, Chungnam National University 2Korea Ocean Reserch & Development Institute |
조직배양에 의한 무지개송어 장기조직세포의 염색체 기본구조에 관한 연구 |
정영래1, 정영길1, 손진기2, 김무강1 |
1충남대학교 수의과대학 2한국해양연구소 |
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Abstract |
This study was examined chromosome structure of kidney, fin, blood cell, sexual organ that were differently examined chromosome of a good cell activity from organ, medium, staining methods, instruments with rainbow trout 100. And so we obtained following conclusion. 1. The difference from each organ and medium is that a good cell activity of fin, kidney, sexual organ were obtained in TC-199 medium and a good cell activity in TC-199 medium+PHA(5%). 2. In the difference by staining methods, G-banding and C-banding were analyzed by electron microscope or cytoscan. Among them, heterochromatin of centromere analyzed by C-banding. 3. The zygotene and the leptotene were analyzed in this study. 4. Karyotype, heterochromatin and each stage of cell division were clearly analyzed by cytoscan. |
Key Words:
chromosome, C-banding, tissue-culture, cytoscan, rainbow trout |
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