Korean Journal of Veterinary Research 1995;35(2):205-216.
Studies of the chromosome structure of tissue cultured different organisms from the rainbow trout
Yung-rae Jung1, Young-gil Jeong1, Jin-ki Son2, Moo-kang Kim1
1College of Veterinary Medicine, Chungnam National University
2Korea Ocean Reserch & Development Institute
조직배양에 의한 무지개송어 장기조직세포의 염색체 기본구조에 관한 연구
정영래1, 정영길1, 손진기2, 김무강1
1충남대학교 수의과대학
2한국해양연구소
Abstract
This study was examined chromosome structure of kidney, fin, blood cell, sexual organ that were differently examined chromosome of a good cell activity from organ, medium, staining methods, instruments with rainbow trout 100. And so we obtained following conclusion. 1. The difference from each organ and medium is that a good cell activity of fin, kidney, sexual organ were obtained in TC-199 medium and a good cell activity in TC-199 medium+PHA(5%). 2. In the difference by staining methods, G-banding and C-banding were analyzed by electron microscope or cytoscan. Among them, heterochromatin of centromere analyzed by C-banding. 3. The zygotene and the leptotene were analyzed in this study. 4. Karyotype, heterochromatin and each stage of cell division were clearly analyzed by cytoscan.
Key Words: chromosome, C-banding, tissue-culture, cytoscan, rainbow trout
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