Cholinergic contraction to the perivascular nerve stimulation on the isolated coronary artery of pig

Shim, Cheol-soo; Park, Sang-eun; Jeon, Seok-cheol; Han, Bang-keun; Kim, Joo-heon

Korean Journal of Veterinary Research 1995;35(2):237-243.

Cheol-soo Shim¹, Sang-eun Park², Seok-cheol Jeon³, Bang-keun Han⁴, Joo-heon Kim⁵

¹Eastern Branch, Gyeongnam Animal Health Institute
²Central Branch, Gyeongnam Animal Health Institute
³Cardiac Center, Masan Koryo General Hospital
⁴College of Veterinary Medicine, Chonnam National University
⁵College of Veterinary Medicine, Gyeongsang National University

돼지 적출 심관상동맥에 있어서 perivascular nerve stimulation에 의한 cholinergic 수축 작용

심철수¹, 박상은², 전석철³, 한방근⁴, 김주헌⁵

¹경상남도 가축위생시험소 동부지소
²경상남도 가축위생소 중부지소
³마산고려병원 심장센터
⁴전남대학교 수의과대학
⁵경상대학교 수의과대학

Abstract

The effects of various autonomic blocking agents to perivascular nerve stimulation were investigated on isolated coronary artery of pig. 1. The magnitude of contractile response to perivascular nerve stimulation increased with increasing frequency(280Hz) of stimulation. 2. The contractions to perivascular nerve stimulation(40V, 40Hz, 0.5msec, 1min) were increased by pretreatment of the cholinestrase inhibitor, physostigmine. 3. The contraction to perivascular nerve stimulation(40V, 40Hz, 0.5msec, 1min) was antagonised by the muscarinic antagonist, atropine. 4. The contraction to perivascular nerve stimulation(40V, 40Hz, 0.5msec, 1min) was blocked by the neural blocker, tetrodotoxin. 5. The contractions to perivascular nerve stimulation(40V, 40Hz, 0.5msec, 1min) were not significantly affected by the ${alpha}$-adrenergic antagonist, phentolamine or ${eta}$-adrenergic antagonist, propranolol. 6. The contractile response by the acetylcholine was increased by the pretreatment of cholinestrase inhibitor, physostigmine. This findings suggest that the powerful excitatory action by the perivascular nerve stimulation may be linked to muscarinic receptor by cholinergic nerve excitation in coronary artery of pig.

Key Words: acetylcholine, physostigmine, perivascular nerve stimulation, coronary artery, pig