Pharmacokinetics of ciprofloxacin in chickens

Kang, Hwan-goo; Cho, Myung-haing; Lee, Hang; Han, Myung-guk; Son, Seong-wan; Kim, Jae-hak; Lee, Jae-jin

Korean Journal of Veterinary Research 1995;35(3):471-480.

Hwan-goo Kang¹, Myung-haing Cho², Hang Lee², Myung-guk Han¹, Seong-wan Son¹, Jae-hak Kim¹, Jae-jin Lee¹

¹Veterinary Research Institute
²College of Veterinary Medicine, Seoul National University

닭에서 ciprofloxacin의 체내 동태에 관한 연구

강환구¹, 조명행², 이항², 한명국¹, 손성완¹, 김재학¹, 이재진¹

¹수의과학연구소
²서울대학교 수의과대학

Abstract

The purpose of this experiment was to develop a simple and reliable HPLC method for the detection of ciprofloxacin in chicken serum and to provide a basic data on pharmacokinetic parameters after oral and intramuscular administration. The results obtained were as follows: 1. 0.2% meta-phosphoric acid: acetonitrile(7:3, v/v) solution had a high and regular recovery rates and was selected as an extraction solution. 2. The recovery rates of ciprofloxacin were 83-97% with the selected solution in chicken serum and the detection limit was 50ng/ml in serum. 3. Ka(abosorption rate constant) were 3.652 1/h in fasted group and 0.880 1/h in non-fasted group, and Ke (elimination rate constant) were 0.061 1/h and 0.133 1/h, respectively. 4. The highest concentration in serum after intramuscular injection was 840ng/ml within 15-30min and 160-324ng/ml in 1.1-3.2 hours after oral administration. 5. The time course of blood concentration fits well into a 2 compartment model. 6. On oral administration of ciprofloxacin with feed, ciprofloxacin was absorbed more slowly and the amount of absorbed was smaller than that of in fasted chickens. 7. Blood concentration of ciprofloxacin increased in a dose-dependent manner after intramusclular and oral administraiton.

Key Words: ciprofloxacin, chickens, HPLC, pharmacokinetics, two compartment model