Pharmacological effects of Artemisia messes-schmidiana var viridis on 1-naphthylisothiocyanate-induced intrahepatic cholestasis in rat

Kim, Kil-soo; Lee, Byeong-noh; Park, Joon-hyoung

Korean Journal of Veterinary Research 1995;35(3):481-488.

Kil-soo Kim¹, Byeong-noh Lee¹, Joon-hyoung Park²

¹Department of Laboratory Animal Research, Asan Institute for Life Sciences
²College of Veterinary Medicine, Kyungpook National University

1-naphthylisothiocyanate에 기인된 랫드의 간내성 담즙분비 정지에 대한 인진호(Artemisia messes-schmidiana var viridis)의 약리학적 효과

김길수¹, 이병노¹, 박준형²

¹아산생명과학연구소 실험동물연구실
²경북대학교 수의과대학

Abstract

In oriental folk medicine, Artemisia messes-schmidiana var viridis(Compositae) has been used for jaundice, hepatitis, diuretic and liver cirrhosis etc. 1-naphthylisothiocyanate(ANIT) has been used for more than 20 years as a model compound to study mechanisms of intrahepatic cholestasis in laboratory animals as rat and mouse. Various biochemical and morphological changes including biliary epithelial and parenchymal cell necrosis occur in the liver of animals treated with ANIT. The purposes of present study are to examine pharmacological effects of Artemisia messes-schmidiana var viridis water extract(AMWE) on alterations of secretion volume and total bile acids level in bile juice, and that of serum AST, ALT, ALP, bilirubin, and glucose levels in rat. AMWE stimulated bile secretion and recovered ANIT-induced cholestasis. Bile acid concentrations increased to more than 60% compared with normal by ANIT, which were returned toward normal value with AMWE treatment. Serum AST and ALT activities were increased by ANIT and yet which were significantly decreased with AMWE treatment. In addition, this effect was apparent in AMWE pretreatment group. Serum glucose levels were increased with AMWE and ANIT, while were decreased compared with control in AMWE posttreatment group. Increased serum total bilirubin contents and ALP activities by ANIT were significantly decreased with AMWE posttreatment. In conclusion, AMWE exerted bile acid-independent choleresis effect and then improved to normal conditions ANIT-induced cholestatic syndromes. Also, AMWE have protective and regenerative effect of hepatocytes in rat.

Key Words: Artemisia messes-schmidiana var viridis water extract, 1-naphthylisothiocyanate, intrahepatic cholestasis, bile acid-independent choleresis