| Comparative analyses of Theileria sergenti isolated from Korea and Japan by southern hybridization and polymerase chain reaction |
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Joon-seok Chae1, Joo-mook Lee1, Oh-deog Kwon1, Seung-ok Lee1, Keon-sang Chae2, Misao Onuma3 |
1College of Veterinary Medicine, Chonbuk National University
2College of Natural Science, Chonbuk National University
3Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University |
| Sourthern hybridization과 중합효소연쇄반응을 이용한 한국과 일본의 Theileria sergenti 비교 |
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채준석1, 이주묵1, 권오덕1, 이승옥1, 채건상2, 3 |
1전북대학교 수의과대학
2전북대학교 자연과학대학
3북해도대학 수의학부 |
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| Abstract |
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The T sergenti DNA fragments used as probes of KTS1(2.4kb) and KTS3(1.5kb) were labeled with digoxigenin-11-dUTP for the Southern hybridization. T sergenti DNAs from different geographic locations(Korea; Chonbuk, Kyungbuk, Chungnam, Kangwon, Cheju island, Japan; Shintoku, Shintoku 9209, Shintoku 9201, Shintoku 9202, Shintoku 9102) which had been digested with Pst I and EcoR I were probed by the digoxigenin-11-dUTP-labeled KTS1 and KTS3. As the results, the samples from Chonbuk, Kyungbuk, Cheju island in Korea and Shintoku, Shintoku 9209, Shintoku 9201, Shintoku 9102 in Japan were positively reacted, but the others from the other locations not reacted. In the comformation test of T sergenti DNA from different geographic locations, all of the samples were positively detected by PCR amplification. |
| Key Words:
Theileria sergenti, Southern hybridization, dot blot hybridization, sequencing, PCR amplification |
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