Korean Journal of Veterinary Research 1996;36(2):337-348.
Subpopulation in periopheral blood leukocyte of Korean native cattle by using monoclonal antibodies specific to bovine leukocyte differentiation antigen
Jin-san Moon1, Yong-ho Park2, Suk-chan Jung1, Bok-gyeong Ku1, Byong-kyu Kang3
1Veterinary Research Institute
2College of Veterinary Medicine, Seoul National University
3College of Veterinary Medicine, Chonnam National University
백혈구 표면항원 특이 단크론항체를 이용한 한우의 말초혈액 백혈구 아군에 관한 연구
문진산1, 박용호2, 정석찬1, 구복경1, 강병규3
1농촌진흥청 수의과학연구소
2서울대학교 수의과대학
3전남대학교 수의과대학
The proportion of leukocyte subpopulation in the host is of a great importance in understanding their functions and disease progress. Many methods have been developed to seperate leukocytes and to measure their activities. Characterization of immune cell subpopulations in Korean native cattle was performed using a set of monoclonal antibodies specific which are specific to bovine leukocyte differentiation antigen. Peripheral blood leukocytes from fifty Korean native and ten Holstein cattle were collected and analyzed for the investigation of leukocyte subpopulation by using monoclonal antibodies and flow cytometry. The result indicated that Korean native cattle have significantly higher proportion of leukocyte subpopulations expressing MHC class II molecules and BoCD4 than Holstein cattle.
Key Words: Korean native cattle, leukocyte differentiation molecules, major histocompatibility complex, cluster differentiation

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