| Effect on lymphocyte subpopulations of Quil A-ISCOMs with recombinant Aujeszkay's disease virus(ADV) gp50, gIII and α-ADV protein |
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Jin-san Moon1, Yong-ho Park2, Suk-chan Jung1, Bok-gyeong Ku1, Sung-il Lee1, Bang-hun Hyun1, Soo-hwan An1, W.C. Davis3 |
1Veterinary Research Institute, RDA
2Seoul National University
3Washington State University |
| 돼지 오제스키바이러스 재조합단백질 gp50, gIII와 α-ADV을 이용한 Quil A-ISCOMs 접종시 백혈구아군 분포율에 관한 연구 |
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문진산1, 박용호2, 정석찬1, 구복경1, 이성일1, 현방훈1, 안수환1, 3 |
1농촌진흥청 수의과학연구소
2서울대학교 수의과대학
3미국 워싱턴 주립대학교 수의과대학 |
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| Abstract |
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An effective candidate subunit vaccine was prepared by using the immunostimulating complexs(ISCOMs) with Quil A and recombinant protein(gp50, gIII and inactive $alpha$-ADV) Aujeszky's disease virus(ADV). The weaned pigs were twice immunized with a ADV-ISCOMs, and followed by intramuscular challenge with $1{ imes}10^4$ $TCID_{50}$ ADV(strain Yangsan). The unvaccinated pigs were also challenged with same dose of ADV. At 5 days after challenge, the control pigs have developed ADV clinical signs. Whereas, the vaccinated pigs protected them from ADV-induced acute symptoms and death. Also, to identify the lymphocyte subpopulation in peripheral blood with pigs from ADV-ISCOMs vaccinated and control group, lymphocyte reacted with a panel of monoclonal antibodies which are specific to swine leukocyte surface antigens and assayed by the flow cytometry. MHC class I, CD2, CD8, N cells, CD11a, and CD45 antigen positive cells were decreased after inoculating virulent ADV Yangsan strain in control group. The data indicated that ISCOMs technique was useful in ADV subunit vaccine preparation and demonstrated the importance of gp50, gIII as a component of ADV vaccine. |
| Key Words:
pig, Aujeszky's disease virus(ADV), Immunostimulating complexs(ISCOMs), Quil A, subunit vaccine |
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