| Isolation of Escherichia coli O157:H7 from animal feces and biochemical characteristics of Verotoxin-2 produced by these strains I. Study on the phages related to production of Verotoxin-2, and Isolation of Escherichia coli O157:H7 from feces of cattle an |
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In-ho Cha1, Yong-hwan Kim2 |
1Pusan Institute of Health and Environment
2College of Veterinary Kyeongsang National University |
| 동물분변에서 Escherichia coli O157:H7의 분리 및 이들 균이 생산하는 Verotoxin-2의 생물화학적 특성 I. 소와 돼지의 분변에서 E coli O157:H7의 분리 및 Verotoxin-2 생산에 관여하여 파아지의 분리에 관하여 |
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차인호1, 김용환2 |
1부산시보건환경연구원
2경상대학교 수의과대학 |
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| Abstract |
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Three and 2 strains of E coli O157 were isolated from fecal materials of cattle (390) and pigs (420) in Korea, respectively. One strain of O157:H7 isolated from cattle and 2 strains of O157:H7 isolated from pigs were identified as verotoxin-1 (VT-1) produing strains and 2 strains (O157:H7 and O157:H-) isolated from cattle were identified as verotoxin-2 (VT-2) producing strains by neutralization test on HeLa and Vero cells. Culture supernatants of the isolates were cytotoxic to HeLa and Vero cells. The levels of cytotoxin produced by isolates were $10^2{sim}10^4$ cytotoxic dose($CD_{50}$)/ml. Also, VT-2-converting bacteriophage was isolated from KSC109 strain which had been isolated from cattle. Molecular weight of the phage DNA was determined as approximately 45 Kb in 0.8% agarose gel electrophoresis, and morphology of the phage stained with phosphotungstic acid was observed by transmissible electron microscopy. |
| Key Words:
E coli 0157, verotoxin, bacteriophage |
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