Identification of Staphylococcus hyicus subsp. hyicus of swine, poultry and bovine origin with the API STAPH system

Park, Cheong-kyu

Korean Journal of Veterinary Research 1996;36(3):657-663.

Cheong-kyu Park

College of Veterinary Medicine, Kyungpook National University

API STAPH system을 이용한 돼지, 닭 및 소유래 Staphylococcus hyicus subsp. hyicus의 동정

박청규

경북대학교 수의과대학

Abstract

The API STAPH system was compared with conventional methods for identification of 214 strains of Staphylococcus hyicus subsp. hyicus isolated from cases of exudative epidermitis in piglets, skin of healthy pigs, skin of healthy chickens and bovine intramammary infections, and biochemical characteristics among the swine, avian and bovine strains were also compared. All of the swine and bovine strains produced acid within 24 hours from fructose, lactose and trehalose by conventional methods, but some of the avian strains showed a delayed positive reaction in these carbohydrates. These delayed positive strains in conventional methods gave usually negative results for them in the API STAPH system. With the API STAPH system, eighteen different profile numbers were encountered in 214 strains of swine, avian and bovine origin. The swine and bovine strains, respectively, were distributed among 4 profiles, while the avian strains were distributed among 17 profiles. The profile number observed most frequently in the strains of each animal species was uniformly 6 516 153. By conventional methods, approximately 96% of the swine strains were positive for ${eta}$-glucuronidase, but not in any strains from chickens and cattle. For hyaluronidase production determined by degradation of sodium hyaluronidate in a solid culture medium, all the swine and bovine strains were positive, but only 37.5% of the avian strains were positive for it. From these findings, there were differences in the production of extracellular active substances between swine strains of Staphylococcus hyicus subsp. hyicus and those isolated from chickens and cattle.

Key Words: swine, bovine, chicken, Staphylococcus hyicus subsp. hyicus, API STAPH system