| Studies on the pathogenesis of Korean isolate of Aujeszky's disease virus in experimentally infected piglets II. Immunohistochemistry and detection of viral nucleic acids by in situ hybridization |
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Woo-young Cho1, Sung-whan Cho2, Choi-gui Park3, Jae-hoon Kim3, Bang-hoon Hyun3, Yong-dhuk Yoon3, Chang-hee Kweon3 |
1Veterinary Service Laboratory, Chungbuk Office of Agriculture and Livestock
2College of Veterinary Medicine, Chungnam National University
3National Veterinary Research Institutue |
| Aujeszky's disease virus 국내분리주 접종자돈의 병리발생에 관한 연구 II. 면역조직화학 및 in situ hybridization 기법을 이용한 항원과 핵산 검출 |
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조우영1, 조성환2, 박최규3, 김재훈3, 현방훈3, 윤용덕3, 권창희3 |
1충청북도 농축산사업소 가축위생과
2충남대학교 수의과대학
3수의과학연구소 |
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| Abstract |
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This study was conducted to elucidate the distribution of Aujeszky's disease viral nucleic acids and antigens in the central nervous system (CNS) of piglets. The first Korean isolate of Aujeszky's disease virus(ADV) that isolated from naturally infected piglets in Yang San, was inoculated into 32 day old piglets with $10^{5.9}TCID_{50}/ml$ through intranasal or intramuscular route. These piglets were sacrificed at every 24hrs for 8 days. The immunohistochemistry (IHC) was conducted to detect the viral antigens in paraffin-embedded tissue sections using avidin-biotin-peroxidase complex (ABC) method. The viral nucleic acids were detected by in situ hybridization (ISH) using ADV specific DNA probe labeled with digoxigenin. The ADV antigens were detected in reticuloendothelial cells of spleen, lymph nodes and tonsil, alveolar walls, leptomeningeal vascular walls, inflammatory foci of each organ, and nerve cells. The viral nucleic acids were detected in the spinal trigeminal nucleus and its tracts of the pons and medulla oblongata by the ISH technique. The pathways of AD viruses in CNS were determined by IHC and ISH. In the intranasally inoculated group, the viruses in nasal mucosa moved to medulla oblongata and pons through the trigeminal nerve. In case of intramuscullarly inoculated group, viruses moved to brain via lymphoid organs or spinal nerves from sciatic nerves. |
| Key Words:
Aujeszky's disease, immunohistochemistry, in situ hybridization, pathogenesis |
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