Immunohistochemical study of the endocrine cells of the gastrointestinal mucosa of the African clawed toad, Xenopus laevis |
Hyeung-sik Lee1, Jae-hyun Lee2 |
1Department of Biology, College of Natural Sciences, Kyungsan University 2College of Veterinary Medicine, Kyungpook National University |
아프리카 발톱두꺼비의 소화관내분비세포에 관한 면역조직화학적 연구 |
이형식1, 이재현2 |
1경산대학교 자연과학대학 생물학과 2경북대학교 수의과대학 |
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Abstract |
The gastrointestinal endocrine cells of the African clawed toad, Xenopus laevis have been investigated immunohistochemically using the avidin-biotin method. Seven antisera were tested and three endocrine cell types immunoreacted with antisera to neurotensin, GRP and substance P. A moderate number of neurotensin-immunoreactive cells were weakly reacted in the small intestine. GRP-immunoreactive cells were mainly situated among the upper portion in the fundic glands, and the basal portion in the pyloric glands. These cells were oval and round in shape. On the other hand, in the intestine they were thin spindly cells with the epithelium. Substance P-immunoreactive cells were observed in among intestinal epithelium. However, no secretin-, motilin-, M-Enk- and PYY-immunoreactive cells were found in the GIT of the African clawed toads. |
Key Words:
endocrine cell, Xenopus laevis, gastrointestinal tract(GIT), immunoreactive cell |
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