Action of P<sub>2X</sub>-purinoceptor on urinary bladder smooth muscle of pig

Park, Sang-eun; Hong, Yong-geun; Shim, Cheol-soo; Jeon, Seok-cheol; Kim, Joo-heon

Korean Journal of Veterinary Research 1997;37(1):103-110.

Action of P_2X-purinoceptor on urinary bladder smooth muscle of pig

Sang-eun Park¹, Yong-geun Hong², Cheol-soo Shim³, Seok-cheol Jeon⁴, Joo-heon Kim²

¹Pusan branch, National Animal Quarantine Service
²College of Veterinary Medicine and Institute of Animal Medicine Gyeong National University
³Eastern branch, Gyeongnam Livestock Promotion Institufe
⁴Cardiac Center, Masan Samsung General Hospital

돼지 방광 평활근에 있어서 P_2X-purinoceptor의 작용

박상은¹, 홍용근², 심철수³, 전석철⁴, 김주헌²

¹국립동물검역소 부산지소
²경상대학교 수의과대학 동물의학연구소
³경상남도 축산진흥연구소 동부지소
⁴마산삼성병원 심장센터

Abstract

The experiments were carried out to elucidate the relationships between neurogenic effects of electrical transmural nerve stimulation and effect of adenosine 5'-triphosphate(ATP) to purinoceptor on the urinary bladder smooth muscle of pig. The results were as follows : 1. The contractile responses induced by electrical transmural nerve stimulation(10V or 20V, 0.5msec, 10sec) were the frequency(2~64Hz) dependent manner. 2. The contractile response induced by carbachol was responsed with a dose-dependent manner and the maximum contractility was $10^{-4}M$. 3. The contractile responses induced by ATP were increased in a dose-dependent manner ($10^{-5}{sim}10^{-3}M$). 4. The contractile response induced by electrical transmural nerve stimulation(10V, 2~32Hz, 0.5msec, 10sec) was partially blocked by the treatment with atropine($10^{-5}M$), and was powerfully inhibited by 3 times of addition with ATP($10^{-5}M$). 5. The contractile response induced by electrical transmural nerve stimulation(10V, 2~32Hz, 0.5msec, 10sec) was partially blocked by the treatment with atropine($10^{-5}M$), and was completely blocked by the desensitization of the $P_{2X}$-purinoceptor using ${alpha}$, ${eta}$-methylene ATP($5{ imes}10^{-5}M$). These results suggest that purinergic nerve was innervated, and ATP and acetylcholine was released by the electrical transmural nerve stimulation in urinary bladder smooth muscle of pig.

Key Words: urinary bladder smooth muscle, ATP, $P_{2X}$-purinoceptor, electrical transmural nerve stimulation