| Comparison of enzyme cytochemical activities between rosetted cells in peripheral blood of Korean native cattle |
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Nyeun-su Kim1, Hee-jong Song2, Sun-jae Kim3, Ye-won Seo1 |
1Chung Bu Branch of the Kang Won-Do Veterinary Service Laboratory
2College of Veterinary Medicine, Chonbuk National University
3Department of Veterinary Medicine, Kon-Kuk University |
| 한우(韓牛) 말초혈액내(末梢血液內) rosette 형성세포(形成細胞)의 효소화학적(酵素化學的) 염색성(染色性) 비교(比較) |
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김연수1, 송희종2, 김순재3, 서예원1 |
1강원도 가축위생시험소 중부지소
2전북대학교 수의과대학
3건국대학교 수의학과 |
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| Abstract |
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Peripheral blood mononuclear cells(PBMNC) of Korean native cattle rosetted with Korean goat erythrocytes(KGRBC) and blood monocytes were evaluated for four cytochemical reactions such as acid phosphatase(ACP), alkaline phosphatase-anti-boby(ALP-Ab), ${alpha}$-naphthyl butyrate esterase(${alpha}$-NBE) and peroxidase. The results obtained were as follows; In rosetted cells, the positivities of ACP in E AET-DeX, EA and EAC were 70.3%, 22.4% and 25.2%, those of ${alpha}$-NB were 27.4%, 44.2% and 79.8%, and those of ALP-Ab were 9.5%, 88.3% and 91.5%, respectively. Whereas, the positivity for Peroxidase in monocytes was 100%. In non-rosetted (remained) cells, the positivities of ACP in E AET+DeX. EA and EAC were 41.4%, 57.2% and 61.9%, those of ${alpha}$-NB were 38.6%, 16.5% and 18.9% and those of ALP-Ab were 98.2%, 5.3% and 6.3%, in order. |
| Key Words:
cytochemical reaction, acid phosphatase, ${alpha}$-naphthyl butyrate esterase alkaline phosphatase, peroxidase |
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