Korean Journal of Veterinary Research 1997;37(1):129-135.
Comparison of enzyme cytochemical activities between rosetted cells in peripheral blood of Korean native cattle
Nyeun-su Kim1, Hee-jong Song2, Sun-jae Kim3, Ye-won Seo1
1Chung Bu Branch of the Kang Won-Do Veterinary Service Laboratory
2College of Veterinary Medicine, Chonbuk National University
3Department of Veterinary Medicine, Kon-Kuk University
한우(韓牛) 말초혈액내(末梢血液內) rosette 형성세포(形成細胞)의 효소화학적(酵素化學的) 염색성(染色性) 비교(比較)
김연수1, 송희종2, 김순재3, 서예원1
1강원도 가축위생시험소 중부지소
2전북대학교 수의과대학
3건국대학교 수의학과
Abstract
Peripheral blood mononuclear cells(PBMNC) of Korean native cattle rosetted with Korean goat erythrocytes(KGRBC) and blood monocytes were evaluated for four cytochemical reactions such as acid phosphatase(ACP), alkaline phosphatase-anti-boby(ALP-Ab), ${alpha}$-naphthyl butyrate esterase(${alpha}$-NBE) and peroxidase. The results obtained were as follows; In rosetted cells, the positivities of ACP in E AET-DeX, EA and EAC were 70.3%, 22.4% and 25.2%, those of ${alpha}$-NB were 27.4%, 44.2% and 79.8%, and those of ALP-Ab were 9.5%, 88.3% and 91.5%, respectively. Whereas, the positivity for Peroxidase in monocytes was 100%. In non-rosetted (remained) cells, the positivities of ACP in E AET+DeX. EA and EAC were 41.4%, 57.2% and 61.9%, those of ${alpha}$-NB were 38.6%, 16.5% and 18.9% and those of ALP-Ab were 98.2%, 5.3% and 6.3%, in order.
Key Words: cytochemical reaction, acid phosphatase, ${alpha}$-naphthyl butyrate esterase alkaline phosphatase, peroxidase


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