Korean Journal of Veterinary Research 1997;37(2):339-347.
Isolation of porcine respiratory coronavirus from pig showing ataxia
Chang-hee Kweon1, Myung-guk Han1, Jae-gil Lee1, Eui-kyung Hwang1, Yung-bai Kang1, Kwang-won Lee2
1National Veterinary Research Institute
2National Animal Breeding Institute
보행실조증 자돈의 뇌조직에서 돼지 호흡기 코로나바이러스의 분리
권창희1, 한명국1, 이재길1, 황의경1, 강영배1, 이광원2
1수의과학연구소
2국립종축원
Abstract
A cytopathogenic virus was isolated from the brain tissues of pig showing ataxia. The biophysical, morphological and serological assay showed that the isolate belongs to a coronavirus. The differential identification of the isolate with monoclonal antibodies against A and X sites of transmissible gastroenteritis virus indicated that the virus has a characteristics of porcine respiratory coronavirus. The RT-PCR on nucleocapsid region of TGEV also showed that the isolate has the same conserved sequence. The diverse pathogenesis of PRCV and its implication in field were discussed.
Key Words: porcine respiratory virus, ataxia, isolation, characterization


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