Isolation of porcine respiratory coronavirus from pig showing ataxia

Kweon, Chang-hee; Han, Myung-guk; Lee, Jae-gil; Hwang, Eui-kyung; Kang, Yung-bai; Lee, Kwang-won

Korean Journal of Veterinary Research 1997;37(2):339-347.

Chang-hee Kweon¹, Myung-guk Han¹, Jae-gil Lee¹, Eui-kyung Hwang¹, Yung-bai Kang¹, Kwang-won Lee²

¹National Veterinary Research Institute
²National Animal Breeding Institute

보행실조증 자돈의 뇌조직에서 돼지 호흡기 코로나바이러스의 분리

권창희¹, 한명국¹, 이재길¹, 황의경¹, 강영배¹, 이광원²

¹수의과학연구소
²국립종축원

Abstract

A cytopathogenic virus was isolated from the brain tissues of pig showing ataxia. The biophysical, morphological and serological assay showed that the isolate belongs to a coronavirus. The differential identification of the isolate with monoclonal antibodies against A and X sites of transmissible gastroenteritis virus indicated that the virus has a characteristics of porcine respiratory coronavirus. The RT-PCR on nucleocapsid region of TGEV also showed that the isolate has the same conserved sequence. The diverse pathogenesis of PRCV and its implication in field were discussed.

Key Words: porcine respiratory virus, ataxia, isolation, characterization