Production of staphylokinase in Staphylococcus hyicus subsp. hyicus strains of swine, poultry and bovine origin

Park, Joon-seo; Park, Cheong-kyu

Korean Journal of Veterinary Research 1997;37(2):359-365.

Joon-seo Park, Cheong-kyu Park

College of Veterinary Medicine, Kyungpook National University

돼지, 닭 및 소유래 Staphylococcus hyicus subsp. hyicus의 staphylokinase 산생능

박준서, 박청규

경북대학교 수의과대학

Abstract

Staphylococcus hyicus subsp. hyicus strains isolated from pigs, chickens and cattle were examined for the production of staphylokinase after inhibition of staphylococcal proteases by two procedures with EDTA(disodium). In one, EDTA was added to the bovine fibrin-dog plasminogen agar medium in concentration of 0.07% and paper strips soaked in 2mg/ml soy bean trypsin inhibitor were then applied on the agar plates. In the other, paper strips soaked in 5% EDTA solution were applied on the bovine fibrin-dog plasminogen agar plates and the strains to be tested were then streaked at right angles with the strip. By these procedures, staphylokinase activity was detected in 8(88.9%) of 9 strains from diseased pigs and in 57(80.3%) of 71 strains from skin of healthy pigs, but not in any strains from skin of healthy chickens and milk samples of mastitic cattle. Additionally kinase activity in 9 Staphylococcus species and subspecies isolated from bovine intramammary infections was also tested by these procedures. Staphylokinase activity was detected in 74.2% of Staph aureus strains and in 25% of Staph xylosus strains.

Key Words: Staphylococcus hyicus subsp. hyicus, staphylokinase, proteases, EDTA