| Cloning and nucleotide sequence analysis of VP7 genes of bovine rotaviruses isolated in Korea |
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Shien-young Kang1, Seong-jin Jeon1, Kyeong-ok Chang2, Yong-ha Park3, Won-yong Kim4 |
1College of Veterinary Medicine, Chungbuk National University
2College of Veterinary Medicine, Ohio State University
3Korea Institute of Science and Technology, Genetic Engineering Research Institute
4College of Medicine, Chungang University |
| 국내에서 분리된 소 로타바이러스의 VP7 유전자 크로닝 및 염기서열 분석 |
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강신영1, 전성진1, 장경옥2, 박용하3, 김원용4 |
1충북대학교 수의과대학
2오하이오주립대학교 수의과대학
3한국과학기술연구원 생명공학연구소
4중앙대학교 의과대학 |
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| Abstract |
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Bovine rotaviruses(A, 288, 55086 strains) isolated from fecal samples in Korea were propagated onto MA104 cells and were confirmed tentatively as G6, G8, and G10, respectively, by RFLP analysis. Full-length VP7 gene of these isolates was amplified by reverse transcriptase polymerase chain reaction(RT-PCR) using VP7 specific primers and cloned into TA vector. Nucleotide and deduced amino acid sequences of VP7 genes of the isolates were determined and compared with those of bovine rotavirus reference strains(NCDV; G6, UK; G6, Cody I-801; G8 and B223; G10). A, 288 and 55086 isolates showed high degree of nucleotide sequence homology with NCDV and UK(93% and 94%), Cody I-801(86%) and B223(97%), respectively, However, they showed 71~74% of nucleotide sequence homlogy with bovine rotavirus reference strains which belong to different serotypes. From the results of deduced amino acid sequence homology analysis, three isolates showed 94~96% of homology with the same serotype reference strains but 80~84% of homology with the different serotype reference strains. Three bovine rotavirus isolates, A, 288 and 55086 strains, were confirmed as G6, G8, and G10, respectively, by nucleotide and deduced amino acid sequence analysis. |
| Key Words:
Bovine rotaviruses, RFLP, G type, VP7 gene sequencing |
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