Effects of purinergic nerve on relaxation of pig coronary artery

Kim, Joo-heon; Shim, Cheol-soo; Jeon, Seok-cheol

Korean Journal of Veterinary Research 1997;37(3):533-540.

Joo-heon Kim¹, Cheol-soo Shim², Seok-cheol Jeon³

¹College of Veterinary Medicine and Institute of Animal Medicine Gyeongsang National University
²Eastern Branch Gyeongnam Livestock Promotion Institute
³Cardiac Center Masan Samsung General Hospital

돼지 심관상동맥의 이완작용에 대한 purinergic 신경의 효과

김주헌¹, 심철수², 전석철³

¹경상대학교 수의과대학, 동물의학연구소
²경남축산진흥연구소 동부지소
³마산삼성병원 심장센타

Abstract

To elucidate the effects of purinergic nerve on relaxation of pig coronary artery, the effects of ATP, 2-methylthio ATP and electrical perivascular nerve stimulation were investigated from physiograph on the isolated coronary artery of pig. The results btained were as follows; 1. The relaxative responses induced by perivascular nerve stimulation(20V, 0.5msec, 10sec) were the frequency(1~8Hz) dependent manner with phentolamine($10^{-5}M$) and atropine($10^{-6}M$) on isolated coronary artery of pig. 2. The relaxative responses induced. by adenosine($10^{-7}{sim}5{ imes}10^{-3}M$) or ATP($10^{-7}{sim}5{ imes}10^{-5}M$) on precontraction with histamine($10^{-5}M$) were the dose-dependent manner, but the contractile responses were often induced by ATP($10^{-4}M$ and $10^{-3}M$). 3. The relaxative responses induced by 2-methylthio ATP($2.5{ imes}10^{-8}{sim}2.5{ imes}10^{-6}M$) on precontraction with histamine($10^{-5}M$) were the dose-dependent manner. 4. The relaxative response induced by 2-methylthio ATP($10^{-7}M$) on precontraction with histamine($10^{-5}M$) was completely blocked by the pretreatment with $P_{2Y}$-purinoceptor blocker, reactive blue 2($10^{-4}M$). 5. The neurogenic relaxative response induced by perivascular nerve stimulation(20V, 8Hz, 0.5msec, 10sec) was weakly inhibited by the pretreatment with ${eta}$-adrenoceptor blocker, propranolol($10^{-5}M$) and blocked by the addition with $P_{2Y}$-purinoceptor blocker, reactive blue 2($10^{-4}M$). The results suggest that the purinergic nerve is innervated, and its relaxative response was mediated by $P_{2Y}$-purinoceptor on isolated coronary artery in pig.

Key Words: 2-methylthio ATP, $P_{2Y}$-purinoceptor, perivascular nerve stimulation, neurogenic relaxation, coronary artery, pig