| Studies on development of ELISA Kits for T-2 toxin |
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Hwa-joong Yoon, Taejong Kim, Sung-yun Lee |
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Department of Veterinary Medicine, College of Animal Husbandry, Kon-Kuk University |
| T-2 독소의 측정을 위한 ELISA Kits의 개발에 관한 연구 |
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윤화중, 김태종, 이승윤 |
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건국대학교 축산대학 수의학과 |
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| Abstract |
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Direct competitive enzyme linked immunosorbent assay(ELISA) with monoclonal antibodies have been studied for quantitative determination of T-2 toxin from the mold corn. The T-2HS, T-2HS-BSA, T-2HS-HRP and monoclonal antibodies against T-2 toxin produced in the studies were qualified for quantitative ELISA test of T-2 toxin. The mean recovery rate from ground com spiked T-2 toxin was 83%. The meaning range of the T-2 test was 60ng to $2{mu}g$. According to the recovery results with the com spiked T-2, the tests proved to be suitable in the screening of the moldy feed samples for the presence of T-2 toxin and will be able to become the basis of the ELISA test for the quantitative screening kits of T-2 toxin. |
| Key Words:
Enzyme Linked Immunosorbent Assay(ELISA), T-2 toxin, T-2HS-BSA, T-2HS-HRP, Monoclonal Antibody |
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