Distribution of neuropeptide-immunoreactive cells of calcitonin gene-related peptide and substance P in the lumbar dorsal root ganglia of WKY rat

Shin, Jae-won; Yoon, Yeo-sung; Won, Moo-ho; Oh, Yang-seok

Korean Journal of Veterinary Research 1997;37(4):719-726.

Jae-won Shin¹, Yeo-sung Yoon¹, Moo-ho Won², Yang-seok Oh³

¹Department of Veterinary Medicine, Kangwon National University
²Department of Anatomy, College of Medicine, Hallym University
³Experimental Animal Center, College of Medicine, Hallym University

WKY 랫드 요수부분 척수신경절 내에서 Calcitonin gene-related peptide와 Substance P 면역반응세포의 분포

신재원¹, 윤여성¹, 원무호², 오양석³

¹강원대학교 수의학과
²한림대학교 의과대학 해부학교실
³한림대학교 의과대학 실험동물부

Abstract

Dorsal root ganglion(DRG) cells are primary sensory neurons which contain some biologically active neuropeptides which play a role as neurotransmitters or neuromodulators. This study was performed to observe normal distribution of calcitonin gene-related peptide (CGRP) and substance P (SP) immunoreactive cells and colocalization of CGRP and SP in a single DRG cell of the lumbar DRGs($L_1{sim}L_6$) in the Wistar Kyoto(WKY) rat by immunohistochemistry. About 55.8% of DRG cells contained CGRP-immunoreactivity, while about 12.7% of DRG cells showed SP-immunoreactivity. There was no significant difference in percentage of each neuropeptied-immunoreactive cells between each neuropeptide-immunoreactive cells between each levels of DRGs ($L_1{sim}L_6$) (p>0.01). In size distribution, CGRP-immunoreactive cells were identified below $1,500{mu}m^2$; SP-immunoreactive cells below $600{mu}m^2$. In serial sections, about 86.7% of the SP immunoreactive cells contained CGRP immunoreactivity.

Key Words: calcitonin gene-related peptide (CGRP), substance P (SP), colocalization, immunohistochemistry, lumbar dorsal root ganglion(DRG)