Effects of cholate and deoxycholate on pancreatic exocrine secretion in sheep

Hyun, Hae-sung; Lee, Chung-gil; Isono, Masanori; Kato, Seiyu

Korean Journal of Veterinary Research 1997;37(4):745-754.

Hae-sung Hyun¹, Chung-gil Lee¹, Masanori Isono², Seiyu Kato²

¹College of Veterinary Medicine, Chonnam National University
²School of Veterinary Medicine, Rakuno Gakuen University

면양의 췌장 외분비 기능에 미치는 cholate 및 deoxycholate의 영향

현해성¹, 이정길¹, 마사노리이소노², 세이유카토²

¹전남대학교 수의과대학
²일본낙농학원대학 수의학부

Abstract

This study was designed to investigate the effects of cholate and deoxycholate on pancreatic exocrine secretion in conscious sheep with external bile and pancreatic fistulae. Bile and pancreatic juices were collected for a basal period of 2 hours. The pancreatic juice was returned to the intestine. Bile salts were infused into the jugular vein or duodenum for 90 minutes at the rate of 0.7mg/kg/min. Cholate and deoxycholate significantly increased the flow rate, pH and bicarbonate concentration of bile juice, but decreased the flow rate of pancreatic juice. The effects induced by intraduodenal infusion of both bile salts were significantly greater than those by intravenous infusion. Protein concentration and amylase activity in pancreatic juice were also significantly decreased by both bile salts; the effects were greater when the bile salts were infused into the duodenum than into the vein. The inhibitory effects induced by deoxycholate infusion were significantly greater than those by cholate infusion. The plasma concentration of secretin was significantly increased by intravenous infusion of deoxycholate, but it was not effected by intraduodenal infusion of both bile salts. The results indicated that cholate and deoxycholate markedly increased the secretion of bile juice and decreased the pancreatic exocrine secretion, although these effects were variable depending on the chemical composition or infusion routes.

Key Words: cholate, deoxycholate, pancreatic exocrine secretion, sheep