Prevalence of pili and enterotoxins of Escherichia coli associated with diarrhea in preweaning piglets

Ham, Hee-jin; Cheon, Doo-sung; Chae, Chan-hee

Korean Journal of Veterinary Research 1997;37(4):779-784.

Hee-jin Ham¹, Doo-sung Cheon², Chan-hee Chae²

¹Seoul Metropolitan Institute of Health and Environment
²College of Veterinary Medicine, Seoul National University

포유자돈 소장에서 분리된 대장균의 섬모항원과 장내독소 분포양상

함희진¹, 천두성², 채찬희²

¹서울시 보건환경연구원
²서울대학교 수의과대학

Abstract

A comprehensive study of 132 Escherichia coli isolates from 150 piglets with colibacillosis included detection of heat-labile enterotoxin, heat-stable enterotoxin, and identification of K88 (F4), K99 (F5), 987P (F6), and F41. Four pili were examined by haemagglutination and slide agglutination test. Heat-labile(LT) and heat-stable(ST) enterotoxin was determined by reverse passive latex agglutination and precipitation test, respectively. Among 132 E coli isolates, 26 had K88 (19.7%), 16 had K99 (12.1%), 3 had 987P (2.3%), and 2 had F41 (1.5%). Three had K88 and K99 (2.3%), 3 had K88 and 987P (2.3%), 2 had K99 and 987P (1.5%), 5 had K99 and F41 (3.8%), and 8 E coli strains had K88, K99 and F41 (6.1%) simultaneously. Among 132 E coli isolates, 5 produced LT only (3.8%), 55 produced heat-stable toxin ST only (41.7%), and 4 produced both LT and ST (3.0%). Three major pathotypes accounted for 27.9% of E coli isolates: $K99^+$ (8.3%), $K88^+ST^+$ (9%) and $K88^+$ (10.6%). Results of this study indicated that piliated enterotoxin-producing E coli was prevalent and was associated with diarrhea in preweaning piglets.

Key Words: Escherichia coli, K88, K99, 987P, F41, heat-labile toxin, heat-stable toxin